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The cultivation of microalgae in bubble column photobioreactors is essential to improve the biomass productivity for biofuel production. This work comprises the design and fabrication of bubble column photobioreactor of 20 L culture volume for the cultivation of  Chlorella pyrenoidosa . The theoretically parameters such as gas hold up and volumetric mass transfer coefficient was predicted. The bubble column photobioreactor of 20 L culture volume was fabricated. The  C. pyrenoidosa  was cultivated in bubble column photobioreactor and the actual gas hold up and volumetric mass transfer coefficient was measured and with predicted values. The results showed similar trend of variation of experimental theoretically predicted gas hold up and volumetric mass transfer coefficient at different gas velocities. The comparison of biomass concentration and lipid content of  C. pyrenoidosa  cultivated in bubble column photobioreactor showed 1.18 ± 0.02 g /L  and 19.04 ± 0.03% dwt, respectively, at the end of the cultivation period as compared with conical flask and glass tank cultivation.

Bubble column photobioreactor for Chlorella pyrenoidosa cultivation and validating gas hold up and volumetric mass transfer coefficient