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Although the role of pancreatic β-cell macroautophagy/autophagy is well known, that of β-cell mitophagy is unclear. We investigated the changes of lysosomal Ca 2+  by mitochondrial or metabolic stress that can modulate TFEB activation and, additionally, the role of TFEB-induced mitophagy in β-cell function. Mitochondrial or metabolic stress induces mitophagy, which is mediated by lysosomal Ca 2+  release, increased cytosolic [Ca 2+ ] and subsequent TFEB activation. Lysosomal Ca 2+  release is replenished by ER → lysosome Ca 2+  refilling through ER Ca 2+  exit channels, which is important for the increase of cytosolic [Ca 2+ ] and mitophagy by mitochondria stressors. High-fat diet (HFD) feeding augments pancreatic β-cell mitophagy, probably as an adaptation to metabolic stress. HFD-induced increase ofβ-cell mitophagy is reduced by  tfeb  KO, leading to increased ROS and decreased mitochondrial complex activity or oxygen consumption in  tfeb -KO islets. In  tfeb  Δβ-cell mice, HFD-induced glucose intolerance and β-cell dysfunction are aggravated. Expression of mitophagy receptor genes including  Optn  or  Calcoco2  is increased by mitochondrial or metabolic stressors in a TFEB-dependent manner, likely contributing to increased mitophagy. These results suggest that lysosomal Ca 2+  release in conjunction with ER → lysosome Ca 2+  refilling is important for TFEB activation and mitophagy induction, which contributes to pancreatic β-cell adaptation to metabolic stress.

Essential role of lysosomal Ca2+-mediated TFEB activation in mitophagy and functional adaptation of pancreatic β-cells to metabolic stress