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This research was carried out with the aim of revealing the genetic differences of 30 cotton varieties from 14 countries using the iPBS-retrotransposon marker method. Genetic differences were determined by using iPBS marker method in PCR for 30 cotton varieties. According to the genetic distance matrix obtained in the NTSYS-pc 2.10e program, the closest genotypes were determined to be AzGR-11468 (Azerbaijan) and Maydos Yerlisi (India), and the similarity value between them was found to be 0.0318. The most distant genotypes were determined to be Hill Cotton 3 (Bangladesh) and Shazbaz (Pakistan), and the similarity value between them was found to be 0.7572. When the dendrogram generated based on the UPGMA method was examined.30 cotton varieties were divided into 2 clades. According to the PCA made in the JMP program, the graphic results and eigen values obtained on the two-dimensional plane also supported these results. The first 3 eigen values explained 85% of the total variance in the population. Overall results suggest that Hill Cotton 3 (Bangladesh) ( Gossypium arboreum ) and Shazbaz (Pakistan) ( Gossypium hirsutum ) cultivars, which are the most distant relatives among 30 cotton genotypes, can be used as breeding cultivars in future cotton breeding studies.

Determining the Genetic Difference of Some World Cotton Genotypes Using iPbs (Inter-Primer Binding Sequences) Retrotransposon Markers