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To compare three different protocols to assess the velocity associated with the maximal oxygen uptake (V max ) in soccer players. Sixteen semi-professional soccer players performed three maximum incremental tests on treadmill: two continuous protocols [1 km·h −1 ·min −1  (CP1); and 1 km·h −1  every 2 min (CP2)], and one discontinuous (DP) protocol to determine V max , maximum oxygen uptake (VO 2max ) and oxygen cost of running (i.e., the slope of the VO 2  vs velocity relationship at submaximal exercise). V max  was higher in CP1&gt; CP2&gt; DP (19.4 ± 1.7, 17.4 ± 1.2, 16.1 ± 1.1 km·h −1  for CP1, CP2, and DP, respectively;  P  &lt; 0.05 ES: 0.09 to 3.36). No difference in VO 2max  was found between CP1, CP2 and DP ( P  &gt; 0.05). Oxygen cost of running showed between-protocol differences (CP1&gt; CP2&gt; DP;  P  &lt; 0.05; ES: 0.28 to 3.30). V max  was higher when determined using continuous vs discontinuous protocols due to the greater overestimation in oxygen cost of running. Such differences in V max  should be considered to optimize acute physiological responses during high-intensity running activities.

Testing protocol affects the velocity at VO 2max in semi-professional soccer players