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We investigated an extraction method of blue fluorescent pigments from Fraxinus lanuginosa (Japanese ash,  aodamo  in Japanese) and found that aodamo contains several fluorescent pigments: not only blue but also red. Reversed-phase column chromatography was performed, and the mushed aodamo’s solution was separated into multiple layers in which the pigments were isolated. Absorbance and photoluminescence measurements indicated that the brown solution first removed from the column contains blue fluorescent pigments and is free of red fluorescent pigments. In addition, the brown solution’s nuclear magnetic resonance (NMR) spectrum clearly suggests that it contains the blue fluorescent pigments of esculin and fraxetin.

Extraction of natural blue emissive pigments from Fraxinus lanuginosa by column chromatography