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The effect of hectorite clay on the monolayer of 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at air-saline interface in presence of fluorescent dye acridine orange (AO) is studied. Surface pressure–area isotherm and compressibility studies reveal the phase behavior and rheological properties of DPPC monolayer. Formation of DPPC/clay complex and their aggregation during monolayer compression partially remove DPPC molecules form the interface. UV-vis absorption and fluorescence emission techniques confirm the aggregation of AO in DPPC/clay/AO hybrid Langmuir-Blodgett film. As phospholipids act as lung surfactants, this model study has implication of realizing the detrimental effects of clay on phospholipids during alveolar surface compression while breathing.

Interaction of hectorite clay with a phospholipid monolayer at air-saline interface in presence of a fluorescent dye