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The rGO/hemoglobin/CdS biocomposite (rGO/Hb/CdS) were prepared  via  a simple route. The water-soluble rGO/CdS composite was synthesized in aqueous solution with a facile hydrothermal route. CdS nanoparticles (NPs) were anchored uniformly on the graphene nanosheets. With L-cysteine (Cys) as the molecular linker, the Hb molecules were then adsorbed onto the CdS NPs to produce the rGO/Hb/CdS biocomposite NPs. The rGO/Hb/CdS was characterized employing UV-vis spectroscopy, powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FE-SEM). The electrochemical behaviors of rGO/Hb/CdS were characterized with cyclic voltammetric technique (CV). A pair of well-defined and quasi-reversible cyclic voltammetric peaks was observed. The cyclic voltammogram demonstrated the direct electron transfer of the Fe(III)/Fe(II) couple of Hb. The glassy carbon electrode (GCE) modified with rGO/Hb/CdS displays an excellent electrocatalytic response to the reduction of H 2 O 2  with a detection limit of 6 × 10 −7  M (S/N = 3). A linear calibration curve can be observed in the range of 1.9 × 10 −6  ∼ 1.5 × 10 −4  M.

The preparation of reduced graphene oxide/hemoglobin/CdS biocomposite and its electrocatalysis for the reduction of hydrogen peroxide