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Microbial bioremediation techniques continued to generate increasing interest due to its cost-effectiveness and being environmentally friendly. In the present study,  Klebsiella variicola  Wt,  K. variicola  MutAc and  K.variicola  MutEb were used to bioremediate Cd 2+  and Pb 2+  simulated contaminated soil in a pot experiment and their genetic variability were determined. The genetic variability among the  Klebsiella variicola  wild type and the mutant strains were investigated using 10 RAPD primers which revealed that 89.7% were polymorphic while 10.3% were monomorphic. The polymorphic information content (PIC) ranged from 0.52–0.86 while the diversity of the gene ranged from 0.56–0.88, this indicated an enormous heterozygosis associated with a high degree of polymorphism and diversity which make  Klebsiella variicola  mutant strains unique. The initial Cd 2+  and Pb 2+  in the simulated soil was 500 and 545 mg/Kg respectively while the Pb 2+  concentration in the unsimulated soil was 45 mg/Kg. it was observed that  K.variicola  MutEb removed 14.6% of Cd 2+  from the unsterilized soils while  K.variicola  MutAc removed 15.6% of Cd 2+ from the sterilized contaminated soils. However,  K.variicola  MutAc removed 27.8% and 11.6% of Pb 2+  from the unsterilized and sterilized metal contaminated soils respectively. The results indicated that the autochthonous bacteria in the soil assisted in the remediation of the contaminated soil, most importantly, it was observed that the mutant strains performed better than the  K.variicola  wild type in terms of bioremediation potential, therefore, the strains could be suitable for the remediation of Cd 2+  and Pb 2+  contaminated soil.

Genetic Variability of Klebsiella Variicola by RAPD-PCR Technique and Bioremoval of Pb 2+ and Cd 2+ from Simulated Contaminated Soils