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In applying bioanalytical approaches, the aim of this study was to determine the toxicity of contaminants derived from a solid waste dumpsite in Calabar (Nigeria), by investigating the alterations of lipid and estrogen signaling pathways in  Poeciliopsis lucida  hepatocellular carcinoma-1 (PLHC-1) cells and compared to  in vivo  African catfish ( Clarias gariepinus ), using polar, nonpolar and elutriate extraction methods. Cells were exposed for 48 hr period to different concentrations of the contaminant extracts. The PLHC-1 cells were evaluated for lipid responses as follows adipoRed assay, retinoid x receptor ( rxr ), peroxisome proliferator-activated receptor isoforms ( ppar -α and γ), estrogen receptor ( er -α) and vitellogenin ( vtg ) transcripts. The lipid signaling activation was also assessed in  vivo  using  C. gariepinus , where hepatic levels of  ppar -α were determined at both transcript and functional proteins levels. Data showed variable-, extract type and concentration-specific elevations in mRNA and protein levels for lipidomic and estrogenic effects. These effects were either biphasic at low and high concentrations, depending upon extract type, or concentration-dependent elevations. In general, these toxicological responses may be attributed to soil organic and inorganic contaminants burden previously derived from the dumpsite. Thus, our data demonstrate a unique lipid and endocrine-disruptive chemical (EDC) effects of each soil extract, suggesting multiple and complex contaminant interactions in the environment and biota. Analysis of numerous soil- or sediment-bound contaminants have numerous limitations and cost implications for developing countries. Our approach provides a bioanalytical protocol and endpoints for measuring the metabolic and EDC effects of complex environmental matrices for ecotoxicological assessment and monitoring.

journal of toxicology and environmental health, part a

A bioanalytical approach for assessing the effects of soil extracts from solid waste dumpsite in Calabar (Nigeria) on lipid and estrogenic signaling of fish  Poeciliopsis lucida  hepatocellular carcinoma-1 cells  in vitro  and  in vivo  African catfish ( Clarias gariepinus )