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The role of multi-heavy metal tolerant bacteria isolated from the rhizosphere of  Eichhornia crassipes  in the phytoremediation of Cu and Pb under laboratory conditions was investigated. The heavy metal tolerant rhizosphere bacteria were identified as  Bacillus cereus ,  Paenibacillus alvei ,  Aeromonas caviae ,  Paenibacillus taiwanensis , and  Achromobacter spanius.  Results showed a significant variation in wet weight, Heterotrophic Plate Count (HPC) of the rhizosphere, HPC of water, removal and uptake of Cu and Pb by  E. crassipes , either alone or in association with the rhizosphere bacteria. The removal of Cu by  E. crassipes  in different experimental conditions showed that OTC (Oxytetracycline) untreated  E. crassipes  with rhizosphere bacteria has maximum removal with 95%, followed by  E. crassipes  alone with 84%. The OTC treated  E. crassipes  with rhizosphere bacteria could remove 81% of Cu. The maximum Pb removal efficiency of 93.4% was shown by OTC untreated  E. crassipes  with rhizosphere bacteria, followed by  E. crassipes  alone with 86.8%. The OTC treated  E. crassipes  with rhizosphere bacteria showed the least removal efficiency with 82.32%. The translocation factor (TF) values for Cu and Pb were lower than 1 indicated that the absorption was mainly accomplished in the roots of  E. crassipes . The order of accumulation of Cu and Pb in  E. crassipes  was noted as root &gt; leaf &gt; petiole.

Role of heavy metal tolerant rhizosphere bacteria in the phytoremediation of Cu and Pb using Eichhornia crassipes (Mart.) Solms