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Phytoremediation technology based on living green plants would clean up water pollution. Through hydroponic experiment, the effects of different concentration of 2, 4-dinitrophenol (2, 4-DNP) on the photosynthetic and chlorophyll fluorescence parameters of  Salix babylonica , and the absorption and purification effect of  S. babylonica  on 2, 4-DNP were measured to explore the tolerance of  S. babylonica  to 2, 4-DNP and the feasibility to purify dinitrophenol waste water by it. The biomass, actual photochemical efficiency (PSII), net photosynthetic rate (Pn), photochemical quenching coefficient (qP), stomatal conductance (Gs), transpiration rate (Tr), maximum photochemical efficiency (Fv/Fm) and chlorophyll content of the  S. babylonica  showed downward trend with the increasing exposure concentrations of 2,4-DNP, but the intercellular CO 2  concentration (Ci) appeared upward trend. Non-photochemical quenching coefficient (NPQ) increased at 5 mg L –1 , then declined with the increase concentrations of 2, 4-DNP. In addition, the percent removal of 2, 4-DNP in 20 mg L –1  waste water was 91.4%. In conclusion, 2, 4-DNP significantly inhibits Pn of  S. babylonica  and the reduction of Pn was caused by decreasing Gs, carboxylation efficiency and chlorophyll content. When the concentration of 2, 4-DNP is not more than 20 mg L –1 ,  S. babylonica  can remove 2, 4-DNP efficiently.

The photosynthetic physiological response and purification effect of Salix babylonica to 2, 4-dinitrophenol wastewater