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The ethanol extract from aerial parts of  Ranunculus  were investigated for its chemical composition by LC-ESI-MS/MS technique, which allowed to identify a series of glycosylated flavonoids and one phenolic acid. RBEE extract showed acetyl-cholinesterase inhibition higher than the reference compound Galantamine at a concentrationof 200 µg/mL. A moderate antibacterial activity of the extract was also obtained against  Staphylococcus aureus  ATCC43300,  Citrobacter freundii  ATCC8090, and  Proteus vulgaris  ATCC29905 at a concentration 100 µg. Additionally, a good reduction in plasma coagulation time at 200 μL was also observed for RBEE.

LC-MS profile, in vitro acetylcholinesterase inhibitory, antibacterial and hemostatic properties of Ranunculus bullatus extract