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Three new compounds, arneatas A-C ( 1 – 3 ), together with three known compounds ( 4 – 6 ) were isolated from the roots of  Arnebia guttata  Bunge. The structures were established on the basis of extensive spectroscopic data including NMR and HRESIMS. All the new compounds ( 1 – 3 ) were tested for their cytotoxic activity against two glioma cell lines (U118-MG and U373-MG)  in vitro  after treatment for 48 h. Compound  1  exhibited moderate cytotoxic activity against U118-MG and U373-MG glioma cell lines, with IC 50  values of 10.4 and 17.5 µM, respectively.

Three new compounds with their anti-glioma effects from the roots of Arnebia guttata Bunge