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Fumigant and contact activity of individual and combination of EOs (cumin seed and black pepper oil) were conducted against rice weevil ( Sitophilus oryzae ). Cumin seed and black pepper oil individually exhibited 100% insecticidal activity (Contact and Fumigant) against  S. oryzae  at 24 and 168 h of exposure in the absence and presence of food, respectively. Overall, differential insect mortality rates were observed with individual EOs; however, cumin seed EO showed higher insecticidal activity in both fumigant and contact bioassays than black pepper EO. Binary mixtures of cumin seed and black pepper oil at 60:40 and 80:20 ratios showed higher toxicity than individual EOs against  S. oryzae  and further, additive interaction of binary combinations of EOs resulted in complete insecticidal activity of  S. oryzae . Thus, we conclude binary mixtures of cumin seed and black pepper EOs might be a promising prophylactic treatment solution for control of  S. oryzae  to prevent stored grain infestation.

Fumigant and contact toxicities of individual and additive combinations of biorational-essential oils for control of rice weevil ( Sitophilus oryzae)