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Hemidesmus indicus  (L.) R. Br. ex Schult. and  Tylophora indica  (Burm. F.) Merrill shoot cultures were treated with different concentrations of yeast extract (YE; 25–200 mg/L) and salicylic acid (SA; 50–200 µM), and their effect on lupeol production was assessed. The maximum dry weight (DW) biomass was recorded when  H. indicus  shoots were treated with SA (50 µM) and  T. indica  shoots with YE (200 mg/L). Highest lupeol yield (335.40 ± 0.04 µg/g DW) was obtained in  H. indicus  shoots after treatment with 50 µM of SA for 3 weeks. Whereas in  T. indica , maximum lupeol content (584.26 ± 8.14 µg/g DW) was recorded by giving treatment with 25 μM of SA for 6 weeks.

natural product research

Elicitor mediated enhancement of shoot biomass and lupeol production in  Hemidesmus indicus  (L.) R. Br. ex. Schult. and  Tylophora indica  (Burm. F.) Merrill using yeast extract and salicylic acid