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Four new  N -phenethylbenzamide derivatives, named piperbetamides A-D ( 1 – 4 ), and six allylbenzene derivatives ( 5 – 10 ) were isolated from the stems of  Piper betle  L. Their structures were determined by HR-ESI-MS and NMR spectroscopic methods. Compounds  1 – 10  were evaluated for their inhibitory effects on the growth of nine microorganisms including five Gram-negative ( Escherichia coli ,  Salmonella enterica  serovar Typhimurium,  Shigella flexneri ,  Pseudomonas aeruginosa , and Extended-spectrum beta-lactam resistant  Klebsiella pneumoniae ), three Gram-positive ( Listeria monocytogenes , Methicilin-resistant  Staphylococcus aureus , Vancomycin-resistant  Enterococcus faecalis ), and one yeast ( Candida albicans ) strains. Compounds  1 ,  3 ,  4 ,  6  and  10  exhibited potential antimicrobial activity against  S. flexneri ,  L. monocytogenes , methicillin-resistant  S. aureus  and vancomycin-resistant  E. faecalis  with minimum inhibitory concentration (MIC) values in the range of 16–32 µg/mL.

Four new N -phenethylbenzamide derivatives from the stems of piper betle and their antimicrobial activity