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Inclusion complexes (ICs) of 2-hydroxypropyl-β-cyclodextrin with the essential oil (EO) from Seculo XXI cultivar of  Psidium guajava  were prepared using kneading (KN) and freeze-drying (FD) methods. The resulting ICs clusters have a nanometric size, with a diameter of approximately 80 and 40 nm for KN and FD, respectively. Complexation efficiency was 80.3% and 50.8% for KN and FD methods, respectively. The larvicidal activity of the EO in DMSO on  A. aegypti  had LC 50  and LC 90  values of 51.49 and 64.51 µg mL −1 , respectively. For the KN method, the toxicity corresponded to 77.54 and 107.29 µg mL −1  for LC 50  and LC 90 , respectively. FD method demonstrated toxicity at concentrations above 600 µg mL −1 . Thus, ICs enable the use of EO in breeding sites for  A. aegypti , thus being potential products to be commercially exploited.

natural product research

Development of inclusion complexes of 2-hydroxypropyl-β-cyclodextrin with  Psidium guajava  L. essential oil by freeze-drying and kneading methods for application as  Aedes aegypti  L. larvicide