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A new spirostanol steroid, aspidiata A ( 1 ), and a new spirostanol steroidal saponin, aspidiata B ( 2 ), along with three known compounds, paris saponin VII ( 3 ), daucosterol ( 4 ), and (25 R )-spirostane-1 β ,2 β ,3 β ,4 β ,5 β ,6 β -hexol ( 5 ), were isolated from whole plants of  Aspidistra triradiata  collected in Vietnam. Their chemical structures were established by spectroscopic analysis and comparison with previously published data. Compound  3  showed strong cytotoxicity against LU-1, Hep-G2, MCF-7, and KB human cancer cell lines with half maximal inhibitory concentration (IC 50 ) values ranging from 0.57 to 1.23 µM. Compound  5  exhibited weak cytotoxic activity against the LU-1 cell line, with an IC 50  value of 95.81 µM.

A new spirostanol steroid and a new spirostanol steroidal saponin from Aspidistra triradiata and their cytotoxic activities