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Tenvermectins A and B are a kind of 16-membered macrocyclic lactone antibiotics with potent insecticidal property, which have been obtained from two genetically engineered strains  Streptomyces avermitilis  MHJ1011 and  S. avermitilis  AVE-H39. Aiming at confirming the absolute configuration of tenvermectin B by X-ray diffraction method, 4″, 5 -di-(2-furoyloxyl) tenvermectin B ( 1 ) was prepared and a suitable crystal of  1  was obtained from MeOH. The absolute configuration of  1  was unambiguously established by single-crystal X-ray diffraction analysis. The determination of the stereochemistry of tenvermectin B could promote its chemical and biological studies as agricultural and veterinary agents. In addition, compound  1  displayed weak acaricidal and nematocidal activities against  Tetranychus cinnabarinus  and  Bursaphelenchus xylophilus  respectively.

Confirmation of the absolute configuration of tenvermectin B by single-crystal X-ray diffraction analysis of its furan formate derivative