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A new oleanane-type triterpene, ardisiapunine  A  ( 1 ), together with eight known compounds were isolated from the roots of  Ardisia lindleyana  D.Dietr. Their chemical structures were determined by means of spectroscopic methods including HR-ESI-MS and (1 D, 2 D) NMR data. The absolute configuration of compound  1  was established by a single-crystal X-ray diffraction experiment. The new compound is an unusual oleanane-type triterpene bearing an acetal and a C-13—C-18 double bond. The cytotoxicity of all isolated compounds were evaluated using four human cancer cell lines, including A549, HepG2, HeLa and U87. The new compound  1  and compound  2  were weakly active but the known compound  6  exhibited a high cytotoxicity compared to cisplatin.

A new oleanane-type triterpene from Ardisia lindleyana D.Dietr and its cytotoxic activity