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The ethanolic extract from leaves of  Rauia resinosa , Rutaceae, provided a new flavone, 5-hydroxy-5’,6,7-trimethoxy-3’,4’-methylenedioxyflavone  (1) , in addition to four known compounds: 3’,4’,5,5’,7-pentamethoxyflavone  (2) , 5,7,8-trimethoxy-3’4’-methylenedioxyflavone  (3) , 3’,4’,5,7,8-pentamethoxyflavone  (4)  and β-sitosterol  (5) . The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR, UPLC-DAD-MS and UPLC-ESI-MS/MS, involving comparison with literature data. Cytotoxicity of leaves and stems extracts, their fractions and compounds ( 2) ,  (3) ,  (4)  and  (5)  were evaluated against T24 (bladder carcinoma), TOV-21-G (ovarian adenocarcinoma) and HepG2 (liver carcinoma) cell lines.

New flavone, and cytotoxicity activity of Rauia resinosa ethanolic extract and constituents