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A new unsaturated fatty glycoside  Humionoactosides  A, together with four known compounds were isolated from the whole plant of  Euphorbia humifusa  Willd. Their structures were identified by MS/MS, NMR and ECD spectroscopic analyses, and comparison with the literature data. All compounds were evaluated for the effect on melanogenesis and tyrosinase activity use B16 cells  in vitro , and the results showed that compound  1  could significantly improve the formation of melanin (138.7%, 50  μ M) and tyrosinase activity (137.2%, 50  μ M) compared with the positive control 8-MOP (125.2%, 138.9%, 50  μ M) in a dose-dependent manner. This is the first time that those compounds reported from  Euphorbia humifusa  Willd. with potential anti-vitiligo activity.

A new anti-vitiligo unsaturated fatty glycoside from Euphorbia humifusa Willd