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Three new ergosterols, colletosterols A-C ( 1 – 3 ), together with two known analogues  4  and  5 , were isolated from the endophytic fungus  Colletotrichum magnisporum  associated with the leaves of  Rauvolfia verticillata  by a bioassay-guided fractionation method. The new structures were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. All the ergosterols were evaluated for their cytotoxic activities against A549 and HeLa cell lines. Compounds  1–3  exhibited notable cytotoxicity with the IC 50  values of 3.76–11.18 μM.

New cytotoxic ergosterols from a plant-associated fungus Colletotrichum magnisporum