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One new anthraquinone, questinlin ( 1 ), four known anthraquinones ( 2–5 ), together with three known benzophenone derivatives ( 6–8 ) were isolated from a wetland fungus  Aspergillus flavipes  PJ03-11. Their structures were elucidated on the basis of detailed spectroscopic analysis, including 1D, 2D NMR (HMQC, HMBC), mass spectrometry, and optical rotation and IR spectra. In addition, the cytotoxic activities against three human cancer cell lines (HepG2, Caco-2 and A549) were evaluated. In this article,  Aspergillus flavipes  PJ03-11, a wetland soil fungus isolated from Honghaitan, Panjin city, Liaoning province, was used to study the secondary metabolites through one strain many compounds (OSMAC) methods. By changing the culture medium and adding the substance stimulating the metabolism of the strain into the fermentation medium, the peak type and amount of ethyl acetate extract in the fermentation medium were studied by HPLC. Finally, 2  m M MgCl 2  was added into the rice solid medium for the expansion fermentation.

7-Hydroxy questin, a new anthraquinone from a wetland fungus Aspergillus flavipes PJ03-11