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The phytochemical investigation of the methanol extract of the stem bark of  Chrysophyllum lacourtianum  led to the isolation and characterization of one new secondary metabolite, lacourtianal ( 1 ), together with eight known compounds. Compounds  2 ;  3 ;  5 ;  6 ;  7  and  9  were reported for the first time from  Chrysophyllum  genus. The structures of compounds  1 – 9  were elucidated on the basis of 1 D and 2 D NMR spectroscopic and mass spectrometric data as well as comparison with the literature. The antibacterial activity of the methanol extract, fractions and compounds  1 – 9  were evaluated against bacterial strains. The methanol extract exhibited moderate activity against  Staphylococcus aureus  NR4674 with MIC values of 500 µg/mL. The  n -hexane fraction showed moderate activity against  Staphylococcus aureus  (ATCC 43300 and ATCC 25923) with MIC values of 125 µg/mL and ursolic acid ( 5 ) exhibited strong activity against  Enterobacter aerogenes  CPC and  Escherichia coli  ATCC 25322 with MIC values of 7.8 and 3.9 µg/mL respectively.

One new constituent from the stem bark of Chrysophyllum lacourtianum De Wild. (sapotaceae)