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Didymella glomerata  and  Truncatella angustata  associated with grapevine trunk diseases (GTDs) in Iran, were grown  in vitro  to evaluate the production of phytotoxic metabolites as potential pathogenicity determinants. 2,5-Dihydroxymethylfuran and (+)-6-hydroxyramulosin were isolated from the culture filtrates of  D. glomerata  and  T. angustata , respectively. They were identified by physical and spectroscopic (essentially 1 D and 2 D  1 H and  13 C NMR and ESIMS) methods and X ray analysis. Both compounds induced significant necrosis and curling on the leaves of the host plant  Vitis vinifera  L. and the effects were concentration dependent. No effect was observed on the leaves of the non-host  Solanumlycopersicum  L.. plant.

Phytotoxins produced by Didymella glomerata and Truncatella angustata , associated with grapevine trunk diseases (GTDs) in Iran