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Extraction methods like maceration, ultrasonication, vortex mixer, soxhlet extraction and microwave assisted extraction (MAE), were evaluated for the extraction of Camptothecin (CPT) from the leaves and stem of  Tabernaemontana alternifolia ,  Tabernaemontana divaricata  and  Tabernaemontana citrifolia . The extracts were analyzed by high performance thin layer chromatography (HPTLC). The results show that the leaves of  Tabernaemontana alternifolia  exhibited highest yield of CPT as compared to the other species. MAE was the most efficient extraction method with CPT extraction yield of 0.154 ± 0.004% w/w from  Tabernaemontana alternifolia  leaves followed by Soxhlet extraction, sonication, maceration, and vortex extraction methods.

Comparison of techniques for the extraction of Camptothecin from Tabernaemontana species