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This study is to develop a method for isolation, identification, and quantitative determination of dammarane-type triterpene saponins in the  Panax notoginseng  fruits (PNF). The saponins were isolated by a serious of chromatographic methods, and their structures were elucidated on the basis of spectroscopic evidence and comparison with those of literature reports. Quantitative assay was performed on an ultra-performance liquid chromatography-UV (UPLC-UV) method. As a result, 22 saponins were isolated from the extract of PNF, among them, compound  1  was a new saponin, named as malonylgypenoside IX, compounds  3 – 10 , and  14–18  were isolated from the PNF for the first time. As to quantitative analysis, the calibration curves showed good linearity ( r  &gt; 0.998) within the concentration range, and the method validation provided good reproducibility and sensitivity for the quantification of eight major saponins with precision and accuracy of less than 3.0%.

Isolation, identification, and quantification of triterpene saponins in the fresh fruits of Panax notoginseng