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Phytochemical investigation on the whole plant of  Chloranthus multistachys  pei (Chloranthaceae) afforded three pairs of new sesquiterpene enantiomers (+)/(−)-chlorantene M [(+)/(−)- 1 ], (+)/(−)-chlorantene M1 [(+)/(−)- 2 ] and (+)/(−)-chlorantene N [(+)/(−)- 3 ]. The structures of new compounds were determined through spectroscopic techniques (HR-ESI-MS, 1 D and 2 D NMR), besides, their absolute and relative configurations were established by using Single-crystal X-ray diffraction analysis and CD spectrum. The anti-inflammatory potential of all compounds was evaluated by applying LPS induced RAW 264.7 macrophage inflammatory model, and the results were that none of these compounds showed activity (IC 50  &gt; 100 μM).

Three pairs of sesquiterpene enantiomers from Chloranthus multistachys pei