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Two new  N -acetyl-ᴅ-glucosamine derivatives, penichryfurans A ( 1 ) and B ( 2 ), were obtained from the fermentation of an endophytic fungus  Penicillium chrysogenum  which inhabited the marine medical red alga  Grateloupia turuturu . Their structures were established by detailed spectroscopic analysis of 1D and 2D NMR in combination with HRESIMS data. The absolute configurations were determined by the (Mo 2 (OAc) 4 )-induced CD and comparison of the calculated and experimental electronic curcular dichroism (ECD) spectra. Both compounds were evaluated for their  in vitro  cytotoxic activities against A549, HeLa, and HepG2 cell lines, among which compound  1  exhibited strong cytotoxicity towards the HepG2 cell line with an IC 50  value of 9.0 μM.

Two new N -acetyl-ᴅ-glucosamine derivatives from the medical algae-derived endophytic fungus Penicillium chrysogenum