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A new flavanone and a new chalcone, brandisianones F and G, were purified from the roots of  Millettia brandisiana  Kurz, moreover, sixteen known compounds were found. The chemical structures of all isolated compounds were identified using spectroscopic methods including 1D-NMR, 2D-NMR, MS, IR and CD data. Chalcone  15  exhibited the most cytotoxic activity against liver cancer, HepG2, and cholangiocarcinoma, KKU-M156, cell lines with IC 50  values of 1.74 ± 0.91 and 1.95 ± 0.95 µg/mL, respectively. Chalcones  2 ,  14 ,  16  and  18  as well as flavones  5 ,  6  and  12  showed moderate cytotoxicity with IC 50  values ranging from 5.39 to 14.00 µg/mL.

Brandisianones F and G from Millettia brandisiana Kurz and their cytotoxicity