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The phloroglucinol eugenial C, eugenial D and eugenial E are the main active compounds in  Eugenia umbelliflora  fruits. This study aims to evaluate the extraction conditions of  E. umbelliflora , using ethanol as solvent, focusing on the phloroglucinol and antimicrobial activity. In order to optimize the extraction conditions, ethanol 50, 70 and 90  o GL was used as a solvent in the proportions of 1:20 (w/v) of drug:solvent ratio (D:S), stirring (330 rpm) at room temperature during 4 h, monitored by LC-UV and antimicrobial assay. The LC-UV method developed was linear over a concentration range of 3.4–68.0, 5.3–106.0 and 5.0–100.8 μg.mL −1  of eugenial C, eugenial D and eugenial E, having LOQ of 1.68, 1.33 and 0.8 μg.mL −1 , respectively. The fruits showed the best herbal raw material and showed the highest phloroglucinol concentration and activity against  S. aureus , when extracted with ethanol 90 o GL, during 4 h, at 1:20 of D:S.

Improvement in phloroglucinol compound extracted from different parts of Eugenia umbelliflora monitored by LC-UV and antimicrobial activity