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The SPME-GC-MS analysis of the aroma components of  Gymnadenia conopsea  subsp.  conopsea , subsp.  densiflora , var.  alpina  and  Gymnadenia odoratissima  var.  odoratissima , var.  idae  were reported. The main components of in total 78 found in  G. conopsea  subsp.  conopsea  were elemicin, cis-9-hexadecenal, hexadecanal, isoelemicin and (Z)-11-hexadecen-1-ol acetate; in subsp.  densiflora  benzyl benzoate, eugenol and trans-isoeugenol; in var.  alpina  benzyl benzoate, methyleugenol and elemicin. In the scent of  G .  odoratissima  var.  odoratissima  were found 2-phenylethyl acetate, eugenol and pentadecane, in var.  idae  mainly C15–C21 alkanes and C16, C18 carbonic acids and some isoprenoid-derivatives. As all tested  Gymnadenia -taxa are allogamous, the differences in scent composition may play a role in pollinator attraction.

Fragrance components of Gymnadenia conopsea and Gymnadenia odoratissima collected at several sites in Italy and Germany