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Two new compounds named trigoflavidus A ( 1 ) and trigoflavidus B ( 2 ), and eight known compounds, trigoflavidone ( 3 ), heterophypene ( 4 ), howpene C ( 5 ), 3,4 -seco- sonderianol ( 6 ), trigonochinene C ( 7 ), fraxidin ( 8 ), isofraxidin ( 9 ), and isofraxetin ( 10 ) were isolated from the leaves of  Trigonostemon flavidus  Gagnep. by various chromatographic methods. Their chemical structures were elucidated via UV, IR, HR-ESI-MS and NMR spectroscopic methods and divided into two groups including six 3,4 -seco- diterpenes ( 1 ,  3 - 7 ) and four coumarins ( 2 ,  8 - 10 ). Absolute configurations at stereocenters of compound  1  were confirmed by comparison of its CD spectra with those of the TD-DFT calculations. At a concentration of 30  µ M, compounds  1 – 10  exhibited weak cytotoxic activity toward LU1, HepG2, MCF7, and SKMel2 human cell lines (cell viability all over 50%).

New 3,4 -seco- diterpene and coumarin derivative from the leaves of Trigonostemon flavidus Gagnep