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The phytochemical constituents in the aqueous methanolic leaf extract of  Triclisia gilletii  responsible for its nephroprotective potentials against ethane-1,2-diol induced nephrolithiasis as previously investigated in our laboratory were elucidated. The extract was prepared using 80% aqueous methanol in 72 h, Phytochemical contents of aqueous methanolic extract of  Triclisia gilletii  (TGME) was identified using both a Thermo Scientific DSQII single quadrupole gas chromatography (GC) and a Thermo Scientific liquid chromatography (LCQ Fleet system) tandem mass spectroscopy. The chromatogram acquisition, detection of mass spectral peaks and their waveform processing were performed using Xcalibur MS Software (Thermo Scientific Inc.). GC-MS analysis revealed the presence of phenols, fatty acids, vitamins and steroids. Likewise, for LC-MS analysis kaempferol and dihydrovomifoliol-O-glucoside were detected. The identified constituents have possible contributively effect on the acclaimed pharmacological potential of  Triclisia gilletii  against ethane-1,2-diol induced nephrolithiasis.

natural product research

Phytochemical profiling of aqeous methanolic leaf extract of  Triclisia gilletii  by gas chromatography (GC/MS) and liquid chromatography (HPLC-PDA–ESI/MS n ) tandem mass spectroscopy (MS): a pointer to its nephroprotection