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Three undescribed prenylated benzoic acid derivatives berberisides A-C ( 1-3 ) and a new clerodane glycoside berberiside D ( 4 ) were isolated from  Berberis tsarica  Aherndt. Their structures were elucidated on the basis of extensive NMR and HR-ESI-MS analysis. The  in vitro  cytotoxic activities of all isolates were studied against lung carcinoma A549, hepatocellular carcinoma HepG2 and breast carcinoma MDA-MB-231 cell lines. Among them, compounds  1  and  4  exhibited anti-proliferative effects against three tumor cell lines with IC 50  ranging from 28.97 ± 2.18 to 35.83 ± 0.72 μM.

Berberisides A-D: three novel prenylated benzoic acid derivatives and a clerodane glycoside from Berberis tsarica aherndt.