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Aiming to valorise the Atlantic Rainforest biodiversity in Santa Catarina, the chemical characterisation of the essential oils (EOs) from leaves of  Vernonanthura montevidensis  (Spreng.) H. Rob. is described for the first time. Fresh leaves collected in the year 2014 and 2015, were submitted to hydrodistillation to give pale blue EOs in yields of 0.21 and 0.19%, respectively. The EOs were characterised by GC-MS and GC-FID semi- and quantitative methods. The monoterpene β-pinene was the major constituent in both samples reaching a maximum of 26.3%. The monoterpene α-pinene and the sesquiterpene β-caryophyllene, were also among the major constituents in both samples. By means of the extracted ion chromatogram procedure, it was possible to detect chamazulene, which was associated with the pale blue colour of the essential oils. In the  in vitro  antimollicute assays, the essential oil was moderately active against  Mycoplasma genitalium  and  M. pneumoniae  with MIC values of 250 µg mL −1 .

Essential oils from leaves of Vernonanthura montevidensis (Spreng.) H. Rob.: chemical profile and antimollicute potential