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Chemical investigation of  Lessingianthus brevifolius  (Less.) H.Rob. aerial parts resulted in the isolation of the hirsutinolide-type sesquiterpene lactones piptocarphol, spicatolide D, piptocarphin D and 8α-acetoxy-10α-hydroxy-13- O -methylhirsutinolide, and also of a cadinanolide identified as 13- O -methylvernojalcanolide 8- O -acetate. Flavonoids, triterpenes and chlorogenic acids were also isolated. In addition, a dereplication study was carried out using UHPLC-HRMS and molecular networking, resulting in the identification of fifteen known compounds, being two sesquiterpene lactones and thirteen flavonoids. Some of the compounds are being described for the first time in  L. brevifolius,  and also in the  Lessingianthus  genus.

Chemical constituents and dereplication study of Lessingianthus brevifolius (Less.) H.Rob. (Asteraceae) by UHPLC-HRMS and molecular networking