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Anthocyanins are good alternative to synthetic dyes for food, pharmaceutical and nutraceutical industries. Owing to their wide occurrence in plant kingdom, an UPLC-ESI-MS/MS method was used to identify and quantify the constituents in flowers of  Ipomoea nil . The qualitative evaluation of  I. nil  results in the characterisation of acylated and non-acylated anthocyanins. Besides characterisation, the total phenolic contents in different fractions of  I. nil  were found to be 49.69 ± 1.74 and 331.54 ± 1.14 mg GAE/g, respectively. The total anthocyanins content was also determined by spectrophotometer and found to be 5.89 mg/100g of cyanidin-3- O -glucoside equivalent. The antioxidant activity of different fraction of  I. nil  was evaluated by different assays (DPPH ● , ABTS ●+  and FRAP). In the direction of natural colour stability, we had studied different stabilising agents/copigments and were found to provide stability up to 140 °C. The extracted anthocyanins were evaluated for acute oral toxicity studies and observed to be non-toxic and may direct the use of  I. nil  for human consumption.

Copigmentation and UPLC-ESI-MS/MS of anthocyanin in Ipomoea nil as potential source of food colorant