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A new abietane-type diterpenoid, dalterpenoid A ( 1 ), a new long-chain alkenone derivative, (3 E ,5 E ,10 E )-8-hydroxytrideca-3,5,10,12-tetraen-2-one ( 2 ), together with six known compounds ( 3 – 8 ), namely  epi -guaidiol A ( 3 ), xylaranol A ( 4 ), daldinone C ( 5 ),  trans -3,4-dihydroxy-3,4-dihydro-anofinic acid ( 6 ), ( R )-6-hydroxymellein ( 7 ), helicascolide A ( 8 ), were obtained from fungus  Daldinia  sp. TJ403-LS1, which was originally isolated from roots of the medicinally valuable plant  Anoectochilus roxburghii . The structures of compounds  1  and  2  were established based on widespread spectroscopic methods, mainly including 1D &amp; 2D NMR and HRESIMS analyses, and the absolute configuration of  1  was further confirmed by electronic circular dichroism (ECD) calculation. All new compounds were tested for the in vitro cytotoxicity against five human cancer cell lines.

A new abietane-type diterpenoid and a new long-chain alkenone from fungus Daldinia sp. TJ403-LS1