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Acclimatisation of orchid seedlings propagated in vitro is a slow process that can be accelerated with the use of plant growth-promoting microorganisms and humic substances. The aim of this study was to evaluate the plant’s response to inoculation with plant growth-promoting bacteria and humic acids, as well as to their combined use, during the period of acclimatisation of  Cymbidium  sp. orchids. The treatments were control; application of humic acids; inoculation with  Stenotrophomonas maltophilia ; inoculation with  Burkholderia cepacia ; inoculation with  S. maltophilia  and  B. cepacia ; inoculation with  S. maltophilia  combined with application of humic acids; inoculation with  B. cepacia  combined with application of humic acids; inoculation with  S. maltophilia  and  B. cepacia  combined with application of humic acids. A total of 150 days after acclimatisation, the plants were biometrically and nutritionally evaluated. Inoculation with the bacterial strains separately promoted seedlings with greater N content; however, only inoculation with  S. maltophilia , resulted in plants with more total dry matter in relation to the control. There was no response from application of humic acids in an isolated manner or in combination with the bacterial strains. The results indicate the biotechnological potential of the bacteria  S. maltophilia  in promoting the growth of the  Cymbidium  sp. orchid.

Acclimatisation of orchids using plant growth-promoting bacteria and humic acids