English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The present research aimed to establish the polyphenolic profile and to appraise the cytotoxic activity first by  in vitro  analyses, followed by a confirmatory  in silico  study to establish a stronger biochemical rationale for the activity of the ethyl acetate extract obtained from the bark of  Tetraclinis articulata . Total phenolics and flavonoids of the ethyl acetate extract were quantified (phenolics, 65.30 mg gallic acid equivalents/g extract; flavonoids, 28.90 mg quercetin equivalents/g extract). A total of 22 polyphenolic compounds were identified in the ethyl acetate extract by HPLC-PDA/ESI-MS analysis.  Tetraclinis articulata  extract exerted a significant cytotoxic effect against a HeLa cell line (IC 50  = 07.02 ± 1.15 μg/mL). The  in silico  analysis was in agreement with the  in vitro  study in which major constituents of ethyl acetate extract showed low binding energy and a correct mode of interaction in the active pocket of the enzyme (PDB: 4I5I). These results might be due to the synergistic action of constituents present in ethyl acetate extract or acting separately. This is the first report on the polyphenolic composition and cytotoxic activity of the extract from the bark of  T. articulata .

plant biosystems - an international journal dealing with all aspects of plant biology

HPLC-PDA/ESI-MS determination of the polyphenolic profile and evaluation of the cytotoxic effect of  Tetraclinis articulata  bark using  in vitro  and  in silico  methods