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The herb holy basil ( Ocimum tenuiflorum ) is rich in essential oil, which is important in pharmaceutical, nutraceutical, cosmetic, and food industries.  O .  tenuiflorum  grows well in tropical, subtropical, and warm temperate regions. To induce essential oil biosynthesis, holy basil plants were exposed to cold or drought stress for 5 days. Gas chromatography-mass spectrometry (GC–MS) analyses revealed that essential oil components including eugenol, methyl eugenol, and β-caryophyllene were significantly accumulated in 30-day-old juvenile and 130-day-old pre-flowering plants. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that expressional levels of  OtEOMT  transcript increased by almost 5-fold following the juvenile stage, and by 16.78- to 66.02-fold following the pre-flowering stage among cold-treated plants. These results suggest a biotechnological strategy to increase essential oil content by treating holy basil plants with cold or drought stress at the juvenile and pre-flowering stages.

Biosynthesis of essential oil compounds in Ocimum tenuiflorum is induced by abiotic stresses