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In this study, the degradability of the antibiotic Ceftriaxone was investigated with the help of genetically engineered  Pseudomonas putida , in which the gene producing the enzyme catechol 2 and 3 dioxygenase was designed and then inserted into the pUC18 plasmid and replicated by  E. coli . It was purified and extracted and transformed into  Pseudomonas putida . Finally, the degradation rate of Ceftriaxone by this bacterium in spiked soil was evaluated using the HPLC measurement technique. Finally, the kinetics of  Ceftriaxone  degradation by genetically engineered  Pseudomonas putida  was investigated using zero, first, and second –order kinetic models for all factors. The results of HPLC measurement showed that the biodegradation of ceftriaxone in spiked soil was significant by genetically engineered  P. putida  compared to autoclaved soil inoculated by wild  P. putida  and normal soil with normal microbial flora ( p  &lt; 0.001) and this bacterium was able to degrade ceftriaxone by 69.53% and kinetic modeling showed that the rate of removal by genetically engineered  Pseudomonas putida  follows the zero-degree reaction model. These findings indicate that  Pseudomonas putida , which produces Catechol 2,3-dioxygenase, can be useful and practical in the biological treatment of environment from cephalosporins.

Biodegradation of ceftriaxone in soil using dioxygenase-producing genetically engineered Pseudomonas putida