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The present study was planned with the objective to isolate and characterize a carbendazim-degrading  Achromobacter  sp. strain GB61 from the contaminated agriculture field. Optimized carbendazim biodegradation (76.2%) by  Achromobacter  sp. strain GB61 was observed at 30 °C, pH 7.0, and 120 rpm within 20 days of incubation and was determined using response surface methodology. The degradation kinetics revealed the half-life ( t 1/2) of 7.3 days, and correlation coefficient ( R 2) 0.0882 and degradation constant ( K ) 0.095 respectively. Maximum specific degradation rate ( qmax ), half-saturation constant ( Ks ) and inhibition constant ( Ki ) were determined to be 0.122 d −1 , 0.724 mg L −1  and 271.71 mg L −1 , respectively. Intermediate metabolites of carbendazim biodegradation confirming the formation of dihydroxybenzimidazole, 2-aminobenzimidazole and benzimidazole as intermediates. The results of the experiments suggested that the strain GB61 could be used for large-scale bioremediation of carbendazim contaminated environments.

Degradation mechanism and kinetics of carbendazim using Achromobacter sp. strain GB61