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Lipase is one of the essential enzymes from the hydrolase family, which can be produced from multiple sources like bacteria, fungi, plants, and animals. Due to the various industrial applications, it is necessary to produce and purify lipase cost-effectively. The present study is concerned with the techno-economic analysis of the production and purification of lipase using  Bacillus subtilis . In the lab experiment, a purification fold of 1347.5 was achieved with 50% recovery upon purification. The experimental data fit into a model, simulate, and economically assess a more extensive industrial setup Using SuperPro designer. Annual production of 64 batches with 26.4 kg of lipase produced per batch, and a total yearly operating cost of $16,021,000, with a payback time of around 1.37 years, were retrieved upon simulating experimental data. This study indicates the potential of the used bacteria for industrial lipase production with its techno-economic feasibility.

Techno-economic analysis of production and purification of lipase from Bacillus subtilis (NCIM 2193)