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Experiments were carried out to illustrate the effect of UV light on the course of the enzymatic reaction of the coumarin derivative. Only the pulsating light of the UV diode gives the correct results for the determination of the kinetic constants of the enzymatic reaction. The enzyme concentration limit was found where the description of the M-M model breaks. It was shown that the system determines the kinetic parameters of enzymatic reactions:  V max—the maximum rate of reaction and  K M — the Michaelis constant. This method produces kinetic constants calculated from the changes in enzyme product concentration using the Michaelis-Menten model. To verify the results, we used a statistical analysis that checks the correctness of the model used.

The influence of UV light on the course of fluorescent enzyme assays