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Conventional fermentation processes need to be upgraded to produce a wide array of biomolecules to overcome lower product yield. The cost of production of biomolecules using the fermentation method could be reduced by increasing the product yield by various process enhancement methods. In this study, different innovative process enhancement methods were evaluated to increase the co-production of uricase and alkaline protease at the bioreactor level. Ultrasound-assisted fermentation (UAF), Extractive fermentation (ATPS), and Ultrasound-assisted extractive fermentation (UATPS) are the three innovative methods used for process enhancement. Maximum enzyme production was obtained in a combinatorial approach of ultrasound and extractive fermentation, i.e., ultrasound-assisted extractive fermentation where uricase and protease production enhanced by 2.5 fold and 1.9 fold, respectively, as compared to conventional fermentation.

Comparative bioreactor studies of different process enhancement methods in B. licheniformis for enzyme co-production