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The purpose of this study was to examine the biochemical properties of African bush mango ( Irvingia gabonensis ) pulp PPO. PPO was purified from  I. gabonensis  fruit pulp in three steps and characterized. A purification fold of 343 with specific activity of 216 U/mg and 13% recovery were obtained as well as molecular weight of 32.67 kDa was observed. The optimum pH and temperature were found to be pH 7.0 and 50 °C respectively while the enzyme showed instability at low pH 2–4 with total inactivation at pH 2 but maximal at pH 5–9 with remaining residual activity of 60–90%, whereas, total enzyme activity inactivation was observed at 90 °C. However, Cu 2+ , Fe 2+  and Mg 2+  enhanced the PPO activity but inhibited by Ca 2+ , Ba 2+ , K +  and Na + . Notably, purified PPO was inactivated completely by urea at concentration above 10 mM while  Km  and  Vmax  values were estimated to be 7.34 mM and 0.36 U/min for catechol, 10.76 mM and 0.30 U/min for  L- DOPA, and 14.90 mM and 0.26 U/min for tyrosine, respectively. The activity of PPO in  I. gabonensis  fruit and its juicy product could be controlled at high temperature in acidified medium.

Biochemical studies of enzyme-induced browning of African bush mango ( Irvingia gabonensis ) fruit pulp