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Purified laccases from bacterial species isolated from marine sediment were applied to degrade Bisphenol A (BPA). The Bacterial species were isolated from marine water sediments sampled from Cove Rock and Bonza Bay beach of the Eastern Cape Province, South Africa was tested for laccase activity on varied phenolic plates. The two most promising strains,  Enterobacter asburiae  ES1 and  Enterobacter  sp. Kamsi was subjected to extracellular laccase production and were identified using molecular methods. Both extracted bacterial laccases showed an affinity for ABTS and PFC substrates and were purified to homogeneity by ammonium sulfate precipitation, anion exchange, and size exclusion chromatography. A specific laccase activity of 231.67 and 218.15 U/mg of protein and a molecular weight of 50 and 55 kDa was obtained from the purified ES1 and Kamsi laccases. Laccase activity was optimum at pH8 and 5 and at 80 °C and 60 °C for ES1 and Kamsi laccases, and they manifested 71.7% and 65.8% BPA decolorizing effects. The optimized treatment condition applied showed maximum BPA removal effects of 85% and 86% at pH7 and 6, while 78% and 79% was degraded at 70 °C and 80 °C while at 250 µL enzyme volume, BPA was actively degraded to 85%, and 75% removal effect showed by ES1 and Kamsi laccases. The molecular identification of the pure colonies using 16S rRNA showed the isolate belonged to the class of gammaproteobacterial. Their nucleotide sequence has been deposited in NCBI with the accession number MN686602 and MN686603. Conclusively, marine habitat serves as a reservoir for active bacterial laccase producers suitable for bioprocess application.

Marine sediment derived bacteria Enterobacter asburiae ES1 and Enterobacter sp. Kamsi produce laccase with high dephenolisation potentials